In biopsy specimens without pathologic glomerular features and in every biopsy specimens of renal allografts from sufferers without recurrent proteinuria , only poor arteriolar immunostaining was observed. Three of five biopsy specimens from sufferers with a diagnosis of minimal-change disease showed granular staining for B7-1 along peripheral capillary walls, indicating a podocyte distribution . B7-1 was absent in specimens from four of five sufferers with secondary FSGS, while weak focal podocyte immunostaining was found in a specimen in one patient .Nevertheless, with emphatic results from such a big cohort research, Dr Choudhary was assured that age the sperm donor was of little significance in couples having sperm donation for fertility treatment. It's sperm quality instead of male age that matters, she said. Regulatory requirements on the utmost age group of sperm donors have a tendency to range between 40 and 45 years. These regulatory thresholds reflect a decline in sperm quality with age group and the greater likelihood of DNA mutations and higher risk of genetic abnormality in offspring. These risks have already been highlighted in many recent studies.